In vivo Brillouin optical microscopy of the human eye
نویسندگان
چکیده
We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, showing the depth-dependent biomechanical properties in the cornea and lens.
منابع مشابه
Variations of the Normal Human Limbal Stem Cells Detected by In Vivo Confocal Microscopy
Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
متن کاملStudy of Optical Models Regarding the Human Eye
Introduction: Until now, many models have been presented for optical study of the human eye. In recent years, surgery on the anterior section of the eye (such as cataract and photo-refractive surgery) has increased, so a study on the optics of the eye and evaluation of vision quality has become more important. Material and Methods: In this article, some of these models are considered. They incl...
متن کاملSpatially-resolved Brillouin spectroscopy reveals biomechanical changes in early ectatic corneal disease and post-crosslinking in vivo
Mounting evidence connects the biomechanical properties of tissues to the development of eye diseases such as keratoconus, a common disease in which the cornea thins and bulges into a conical shape. However, measuring biomechanical changes in vivo with sufficient sensitivity for disease detection has proved challenging. Here, we present a first largescale study (~200 subjects, including normal ...
متن کاملIn vivo measurement of age-related stiffening in the crystalline lens by Brillouin optical microscopy.
The biophysical and biomechanical properties of the crystalline lens (e.g., viscoelasticity) have long been implicated in accommodation and vision problems, such as presbyopia and cataracts. However, it has been difficult to measure such parameters noninvasively. Here, we used in vivo Brillouin optical microscopy to characterize material acoustic properties at GHz frequency and measure the long...
متن کاملSynthesis and characterization of CdO/GrO nanolayer for in vivo imaging
Objective(s): Nanomaterials are playing major roles in imaging by delivering large imaging payloads, yielding improved sensitivity. Nanoparticles have enabled significant advances in pre-clinical cancer research as drug delivery vectors. Inorganic nanoparticles such as CdO/GrO nanoparticles have novel optical properties that can be used to optimize the signal-to-background ratio. This paper rep...
متن کامل